Application Note: Macrophage Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and s suitable as a control for polyclonal or monoclonal anti-Macrophage Colony Stimulating Factor in immunological assays. Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0.
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein. Biologic Activity: The activity is determined by the dose-dependent proliferation of mouse NFS-60 cells and is typically 10.0 ng/mL.
Physical State: Lyophilized
Purity and Specificity: Macrophage Colony Stimulating Factor purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Background: Macrophage Colony Stimulating Factor (M-CSF) is a hematopoietic growth factor produced by a wide variety of cells. M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant human M-CSF is a disulfide linked homodimer, containing two 159 amino acid chains, with a total molecular weight of 36.8 kDa.
Low Endotoxin: Yes
Other: User Optimized
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