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Encompassing Amino Acids: 1-327
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process. TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1.
Description: Human RIPK1, also known as RCK and MAP3K19, GenBank Accession No. NM_003804, a.a. 1-327, with N-terminal GST-tag, MW=63 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_003804
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -70°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal GST-tag
Uniprot: Q13546
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hsu, H. et al., Immunity 4: 387-396, 1996. 2. Kim, J Y. et al., J Cell Sci. 2011 Feb 15;124(Pt 4):647-56.
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