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Encompassing Amino Acids: 5-554
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element (1). ROCK2 is an immediate downstream target of the small GTPase RhoA. ROCK2 may play a pivotal role in cardiovascular diseases such as vasospastic angina, ischemic stroke, and heart failure. Inhibition of ROCKs by statins or other selective inhibitors leads to the upregulation and activation of endothelial nitric oxide synthase (eNOS) and reduction of vascular inflammation and atherosclerosis (2).
Description: Recombinant human ROCK2 (a.a. 5-554) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_004850.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_004850
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O75116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhao, Y M. et al. Dev Biol. 2004 Nov 1;275(1):183-91.
2. Noma, K. et al. Am J Physiol Cell Physiol. 2006 Mar;290(3):C661-8.
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