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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: ROCK2, an isoform of ROCK1, is a serine/threonine kinase target of small GTPase Rho that regulates the formation of actin stress fibers and focal adhesions, cytokinesis, and smooth muscle contraction. It is therefore important to proper vascular function and is involved in a number of fibrotic pathologies. The ROCK2 inhibitor Fasudil has been investigated for the treatment of cerebral cavernous malformation (CCM), which causes brain hemorrhages, and is in clinical trials for other applications such as Amyotrophic Lateral Sclerosis (ALS). Belumosudil, a newer ROCK2 inhibitor, was approved by the FDA for chronic graft versus host disease in 2021.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The ROCK2 Kinase Assay Kit is designed to measure ROCK2 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant ROCK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Uniprot: O75116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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