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Encompassing Amino Acids: 429-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM beta-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate ROR1 with 0.25 mg/ml Myelin BasicProtein substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: ROR1 is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors that play a role in the control of most basic cellular processes including cell proliferation, differentiation, migration and metabolism (1). Many human breast cancers express the ROR1 protein and high-level expression of ROR1 in breast adenocarcinoma is associated with aggressive disease. Silencing expression of ROR1 in human breast cancer cell lines found to express this protein impaired their growth in vitro and also in immune-deficient mice (2).
Description: Recombinant human ROR1 (GenBank Accession No. NM_005012), a.a. 429-end, with N-terminal GST-tag, MW 82 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Genbank: NM_005012
Host Cell Line: Sf9 cells
Purity: ≥95%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q01973
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Afzal, A. R. et.al. Hum. Mutat. 22: 1-11, 2003.
2. Zhang, S. et al. PLoS One. 2012;7(3):e31127.
3. Channavajhala, P., et al., J. Biol. Chem. 2003,278:47089-47097.
4. Rodriguez, C., et al., Cell Signal 2006,18(9):1376-1385.
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