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Encompassing Amino Acids: 401-550
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human SAP145, a substrate for PRMT9, also known as Splicing Factor 3b Subunit 2 (SF3B2), (GenBank accession No. NM_006842), (a.a. 401-550) with an N-terminal GST-tag, MW= 45kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 16mM glutathione, 20% glycerol
Genbank: NM_006842
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13435
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Yang, Y., et al. (2015) Nat Commun 6, 64282. Falk, S., et al (2016) Nat Commun 7, 13573
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