SBE Reporter Kit (TGFβ/SMAD signaling pathway)

Products from BPS Bioscience require a minimum order value above 400€

Applications: Monitor TGFβ signaling pathway activity.
Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.

Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

Format: assay kit

Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Supplied As: Reporter: SBE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Moustakas A et al. (2001) Smad regulation in TGF-beta signal transduction. J.Cell Science. 114(Pt 24): 4359-69.

• SKU: BPS60654
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 60654
• Green Labware: No
• Package Unit: 500 rxns.
• Quantity Unit: PAK