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Application Note: This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.
Concentration Value: 80 mg/mL
ELISA Dilution: User Optimized
Western Blot Dilution: 1:5,000 to 1:8,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This Bat3 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/Bat3.
Physical State: Liquid (sterile filtered)
Purity and Specificity: This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01%. The antibody detects endogenous Scythe/Bat3 in cell lysates.
Background: Scythe protein (also known as Bat3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. Scythe binds Reaper, a potent apoptotic inducer. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. Scythe interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. Scythe and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. HLA-B-associated transcript 3 (Bat3/Scythe) is essential for p300-mediated acetylation of p53. Bat3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.
Low Endotoxin: No
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