Formulation: Lyophilized from sterile PBS, pH 7.4
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Semaphorin-3A (Sema3A) is a secreted glycoprotein and ligand of neuropilin-1 (NRP-1) and plexin A receptors.{64856,64857} It is composed of a Sema domain, which mediates signaling and receptor specificity, a plexin-semaphorin-integrin (PSI) domain, an immunoglobulin (Ig) domain, and a C-terminal domain, which contains several basic residues that are important for NRP-1 binding. Sema3A is expressed in many tissues, including nervous, bone, and connective tissues and vascular endothelium, as well as some immune cells such as T cells and macrophages.{64856} It is involved in axonal guidance, bone remodeling, immunoregulation, and angiogenesis.{64856,64857,19718} Sema3A decreases the length and number of neurites in sensory and motor neurons in vitro and increases vascular permeability in mice.{64858,64859} Heterozygous deletion of SEMA3A is associated with Kallmann syndrome, a disorder characterized by hypogonadotropic hypogonadism and an absent or decreased sense of smell.{64860} Cayman’s Sema3A Sema Domain (human, recombinant; aa 26-546) protein is a disulfide-linked homodimer. The reduced monomer, composed of Sema3A (amino acids 26-546) fused to human IgG1 Fc at its N-terminus, consists of 781 amino acids and has a calculated molecular weight of 87.7 kDa.
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