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Encompassing Amino Acids: 2-551(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. Sestrin 1 mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mTOR protein. Sestrin 1 in important for antioxidant defense because it regenerates overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation.
Description: Human Sestrin1, also known as p53-regulated protein PA26 (GenBank Accession No. NM_014454), full length, a.a. 2-551(end), with N-terminal FLAG-tag, MW=65 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 90 ?g/ml FLAG peptide and 10% glycerol
Genbank: NM_014454
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q9Y6P5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lee, J.H., et al. (2010). Science 327(5970): 1223-8.
2. Broderick, G., et al., (2006). Pharmacogenomics 7(3): 407-419.
3. Budanov AV, Karin M. (2008). Cell 134 (3): 451-460.
4. Hay, N. (2008). Cell Metab. 8(3):184-5.
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