Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 52-266 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl
2, 4 mM DTT, 20 µM S-adenosyl-methionine, and 0-10 ng SET7/9) add to the wells coated with the substrate. Incubate at room temp for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence.
Background: SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.
Description: Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal GST-tag, MW = 61 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_030648
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8WTS6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ivanov G.S. et al., Mol. Cell. Biol. 27 (19), 6756-6769 (2007).
2. Couture J.F. et al., Nat. Struct. Mol. Biol. 13 (2), 140-146 (2006).Application Reference(s):
Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)
English
Deutsch
