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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: SETD2 is a histone methyltransferase that exhibits methylation activity toward histones H3-K36
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2activity for screening and profiling applications. The key to the SETD2Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36residue of Histone H3. With this kit, only three simple steps on a microtiter plate arerequired for methyltransferase detection. First, S-adenosylmethionine is incubated with asample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibodyfollowed by addition of the HRP substrate to produce chemiluminescence that can then bemeasured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient strip format, with a 96-well plate precoated with histone H3 peptide substrate, purified SetD2 and reagents for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
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