Background: EBNA-3A is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA-3A functions as a transcriptional regulator though the target genes are currently unknown. Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop
Positive Control: EBV infected tissues and cells
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Source: Sheep were immunized with recombinant EBNA-3A protein
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
References: Bhattacharjee, Shaoni & Bose, Priyanka & Patel, Krishna & Ghosh Roy, Shatadru & Gain, Chandrima & Gowda, Harsha & Robertson, Erle & Saha, Abhik. (2018). Transcriptional and epigenetic modulation of autophagy promotes EBV oncoprotein EBNA3C induced B-cell survival. Cell Death & Disease. 9. 10.1038/s41419-018-0668-9.Anderton, E., et al, 'Two Epstein-Barr virus (EBV) oncoproteins cooperate to repress expression of the proapoptotic tumour-suppressor Bim: clues to the pathogenesis of Burkitt's lymphoma' Oncogene 2007, 27, , 421-432O'Nions, J., et al, 'Epstein–Barr virus can inhibit genotoxin-induced G1 arrest downstream of p53 by preventing the inactivation of CDK2' Oncogene 2003, 22, , 7181-7191Jiménez-Ramírez, C., et al, 'Epstein-Barr Virus EBNA-3C Is Targeted to and Regulates Expression from the Bidirectional LMP-1/2B Promoter' Journal of Virology 2006, 80, , 11200-11208Yuan, J., et al, 'Virus and Cell RNAs Expressed during Epstein-Barr Virus Replication' Journal of Virology 2006, 80, , 2548-25655. Hong, G.K., et al, 'Epstein-Barr Virus Lytic Infection Contributes to Lymphoproliferative Disease in a SCID Mouse Model' Journal of Virology 2005, 79, , 13993-14003Rosendorff, A., et al, 'EBNA3C Coactivation with EBNA2 Requires a SUMO Homology Domain' Journal of Virology 2004, 78, , 367-3777. Maruo, S., et al, 'Epstein-Barr Virus Nuclear Protein EBNA3A Is Critical for Maintaining Lymphoblastoid Cell Line Growth' Journal of Virology 2003, 77, , 10437-10447Dalbiès-Tran, R., et a, 'Amino Acids of Epstein-Barr Virus Nuclear Antigen 3A Essential for Repression of Jkappa -Mediated Transcription and Their Evolutionary Conservation' Journal of Virology 2001, 75, , 90-99 Sample, J., et al. ‘Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes.’ J. Virol., 64, 4084-4092 (1990)
UniProt: Q69138 (EBV/HHV4)
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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