Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to alpha-1 antichymotrypsin. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of alpha-1 antichymotrypsin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Source: Alpha-1 antichymotrypsin is a protease inhibitor with a molecular weight of 68.000. Its concentration in fetal human serum is low at birth, but the concentration increases rapidly to normal plasma levels of about is 4.5 μg/ml. Alpha-1 antichymotrypsin is regulated like a an acute phase reactant. It has been isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Precipitating polyclonal Sheep antiSerum to Human alpha-1 antichymotrypsin.
Formulation: Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added. Total protein and IgG concentration are comparable to those of pooled normal sheep serum.
UniProt: P01011
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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