Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to factor I. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human EDTA plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of F I or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Source: Factor I is a serine protease composed of two disulphide-linked chains. The protein has a molecular weight of 88.000 and its mean concentration in human plasma is 35 μg/ml. It is involved in the regulation of the C3/C5 convertase of either pathway. Using C4-binding protein or factor H splits the a chains of C4b or C3b respectively, which includes their roles in the C3/C5 convertases. It has been isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Precipitating polyclonal Sheep antiSerum to Human factor I.
Formulation: Delipidated, heat inactivated, lyophilized, stable IgG fraction. No preservative added. Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added.
UniProt: P05156
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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