Clone: Polyclonal
Background: The reactivity of the antiserum is directed to the Fc subunit of the IgD molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgD of BALB/C and C57Bl origin used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Pools of purified homogenous IgD isolated from BALB/C and C57Bl mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Sheep antiSerum to Mouse IgD, Fc specific.
Formulation: FITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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