Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In immunocytochemical and immunohistochemical staining for the detection of IgG2a(1b) at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure IgG2a(1b) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labelled antibody may by itself cause high unspecific background staining or interfere with the specific signal.
Source: Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Sheep antiSerum to Mouse IgG2a(1b), allotype specific.
Formulation: Fluorochrome-coupled purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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