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Applications: Great for studying enzyme kinetics and HTS applications.
Background: Mammalian PTPases can be subdivided into 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. SHP-2 (PTPN11) is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
Description: The Homogeneous SHP-2 Assay Kit is a complete assay system designed to measure SHP-2 activity for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is needed to analyze the SHP-2 activity level. The fluorogenic substrate, DiFMUP, is incubated with purified SHP-2 and the enzymatic activity releases DiFMU fluorophore that can then be measured using a fluorescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SHP-2 activity measurements. In addition, the kit includes purified SHP-2 enzyme and SHP-2 inhibitor, orthovanadate, for use as a positive and negative control.
Biosafety Level: Not applicable (BSL-1)
References: Chai, J., et al., Cell, 2001 Mar 9;104(5):769-80.
Denault, JB., and Salvesen, GS., J. Biol. Chem., 2003 Sep 5;278(36):34042-50.
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