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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 200 µM SIRT5 substrate 5 (Catalog #50401), 500 µM NAD+ and Sirtuin 5 (15.6 - 1000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Description: Human Sirtuin 5 (GenBank Accession No. NM_012241), full length with N-terminal GST-tag, MW= 59.8 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, and 10% glycerol
Genbank: NM_012241
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9NXA8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mahlknecht, U. et al., (2006) Cytogenet.Genome Res. 112, 208-212.
2. Frye, R.A. (2000) Biochem. Biophys. Res.Commun. 273, 793-798.
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