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Applications: Drug compound screening, Functional assays
Background: Iodine transport is essential for the production of thyroid hormone (T4). Sodium IodideSymporter SLC5A5 (NIS) is a sodium-dependent transporter of iodide into thyroid follicular cellsand other peripheral tissues to support T4 synthesis [1]. Lack of iodide transport can result inhypothyroidism and can manifest itself in several disease states like goiter formation and weightgain. SLC5A5 also plays an important role in hyperthyroidism (an excessive production of T4).Expression and activity of SCL5A5 is altered in several forms of cancer. Inhibitors of SLC5A5have potential therapeutic benefit for the regulation of iodine overload induced by amiodaroneor other thyromimetics that can result in hyperthyroidism [2].
Description: Stable recombinant HEK293 cell line expressing human SLC5A5 (UniProt # Q92911) with a C-terminalFLAG- tag. SLC5A5 is a sodium-dependent iodide transporter and is a member of thesodium glucose co-transporter family. The expression of SLC5A5 is confirmed by Westernblotting. This cell line can be used to evaluate agents that may affect iodide transport.
Format: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Host Cell Line: HEK293
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Uniprot: Q92911
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Dohan et al., (2003) The sodium/iodide Symporter (NIS): characterization, regulation,
and medical significance. Endocrine Reviews 24(1):48-77.
2. Lindenthal et al., (2009) Characterization of NIS inhibitors. Journal of Endocrinology
200: 357-365.
3. Waltz et al., (2010) A nonradioactive iodide uptake assay for sodium iodide
symporter function. Analytical Biochemistry 396(1): 91-5.
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