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Encompassing Amino Acids: 1375-1511
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Description: Human Bromodomain SMARCA2 or BRM, a.a. 1375 - 1511 (GenBank Accession No. NM_003070) with N-terminal Avi-His-tag, MW = 19 kDa, expressed in an E. coli expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.
Genbank: NM_003070
Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Tags: N-terminal Avi-His-tag
Uniprot: P51531
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Shain, A.H., et al., Proc Natl Acad Sci USA. 2012 Jan 31; 109(5): E252-9.2.Liu, G., et al., Oncogene. 2011 Jul 21; 30(29): 3295-304.
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