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Encompassing Amino Acids: 130-end
Applications: Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF2 (0-2 µg) in 50 mM Tris pH 7.4) incubate for 30 min. at 37°C, then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against SMURF2, incubate 1 hr. Finally, incubate 1 hr. with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (λexc=330 nm, λem=620 nm).
Description: Human SMURF2 (GenBank Accession No. NM_022739), a.a. 130-end, with N-terminal FLAG-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Genbank: NM_022739
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q9HAU4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Narimatsu M. et al., Cell 137 (2), 295-307 (2009)
2. Fukunaga E. et al., J. Biol. Chem. 283 (51), 35660-35667 (2008).
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