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Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif. It catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan, which is an essential procedure for pathogens to sustain infection. Thus, Sortase A has potential as an anti-infective therapy target, especially for antibiotic-resistant bacteria.
Description: The Sortase A Assay Kit is designed to measure Sortase A activity for screening and profiling applications.
Storage Stability: Up to 6 months when stored as recommended.
Supplied As: The Sortase A Assay Kit comes in a convenient 96-well format, with enough purified recombinant Sortase A, Triglycine, Abz/Dnp substrate, and assay buffer for 96 enzyme reactions.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mazmanian, S.K., et al. 1999. "Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall." Science 285(5428): 760-763.
2. Spirig, T., et al. 2011. "Sortase enzymes in Gram-positive bacteria." Molecular microbiology 82(5): 1044-1059.
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