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Encompassing Amino Acids: 1-127(full length)
Applications: ELISA and neutralization assays.
Assay Conditions: Inhibition of ACE2:SARS-CoV-1 Spike binding by a small anti-SARS-CoV-1 Spike antibody, VHH (BPS Bioscience, #100784). A small anti-SARS-CoV-1 Spike antibody was evaluated using the SARS-CoV-1 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience, #79999). The antibody was serially diluted in 1x Immuno Buffer (BPS Bioscience, #79311) and tested following the assay kit protocol.
Description: SARS-CoV monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. VHHs are single-domain antibodies from camelids.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Protein A Chromatography
Storage Stability: Stable for at least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Fc-Avi-Tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ho, M. et al., Antibody Therapeutics. 2020; 3(2): 109-114
2. Wrapp, D. et al., 2020. Cell 181: 1004-1015
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