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Applications: ELISA and neutralization assays.
Assay Conditions: Spike Trimer (S1+S2), His-tag (SARS-CoV-1) (BPS Bioscience, #100789) and Spike S1 Neutralizing Antibody (VHH), Fc-fusion (IgG1), Avi-Tag (SARS-CoV-1),Biotin-labeled binding was measured by ELISA using Strepavidin-HRP and Colorimetric HRP substrate.
Description: SARS-CoV-1 monoclonal Spike S1 VHH neutralizing antibody, a.a. 1-127(full length), with C-terminal Avi-Tag fused to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%. VHHs are single-domain antibodies from camelids.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Protein A Chromatography
Purity: ≥90%
Storage Stability: Stable for at least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ho, M. et al., Antibody Therapeutics. 2020; 3(2): 109-114
2. Wrapp, D. et al., 2020. Cell 181: 1004-1015