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Encompassing Amino Acids: 1-1213
Description: Severe acute respiratory Coronavirus Spike trimer (S1+S2), with 682-685>A, K986P, V987P, and D614G mutations, Genbank Accession No. MN908947, a.a. 1-1213, with a C-terminal His-tag, expressed in a HEK293 expression system. MW=137 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: MN908947
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Uniprot: P0DTC2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Klumpp-Thomas, C., et al., MedRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.08.20147371
2. Yurkovetskiy, L., et al., bioRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.04.187757
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