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Description: Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV2 Variant P.1 originally discovered in Brazil and contains 11 mutations in addition to 682RRAR685>A, K986P and V987P, as listed below. The construct also contains a C-terminal His-tag. Note that the expected MW of the S1+S2 monomer is 136kDa but migrates at a higher MW in SDS-PAGE due to glycosylation. The recombinant protein is ≥90% pure following high affinity Ni-NTA purification.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Genbank: MN908947
Host Cell Line: 293 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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