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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200 µM ATP, 2 µM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.
Description: Human SRC (GenBank Accession No. NM_005417), full length with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT.
Genbank: NM_005417
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P12931
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Finlay, D. and Vuori, K. Cancer Res. 67 (24), 11704-11711 (2007).
2 Vielreicher, M. et al. J. Biol. Chem. 282 (46), 33623-33631 (2007).
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