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Applications: Monitor STAT3 pathway activity. Screen activators or inhibitors of the STAT3 signaling pathway. Study effects of RNAi or gene overexpression on the activity of the STAT3 pathway.
Background: STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor which is activated through phosphorylation at Tyrosine 705 in response to various cytokines and growth factors. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1 and the dimers translocated to the nucleus where DNA binding/promoter induction occurs. STAT3 plays an important role in cellular processes such as cell cycle progression and apoptosis.
Description: The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Storage Stability: Stable for at least 12 months at -20°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tian S., et al., Blood. 1994; 84(6):1760-1764.2. Zhong, Z., et al., Science. 1994; 264(5155):95-98.
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