Application Note: STAT4 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. STAT4 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~87kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 0.2 µg/µL
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant full-length human STAT4 was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
Background: STAT4 (signal transducer and activator of transcription 4) is a member of the STAT family that is phosphorylated by receptor-associated protein kinases. STATs form homo- or heterodimers and translocate to the cell nucleus where they act as transcription activators. T helper (Th) cells differentiation is regulated by STAT4 (1). STAT4 controls T1/T2 balance during the response to live influenza virus and isolated viral proteins. The differentiation of gamma interferon (IFN-gamma)-producing Th1 and Tc1 cells after inoculation of live virus occurred independently of STAT4 expression. STAT4 has been shown to control pathogenesis of chronic obstructive pulmonary disease (2). STAT4 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, and JAK/STAT Pathway research.
Low Endotoxin: No
Other: Kinase Assay-User Optimized
English
Deutsch
