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Applications: Monitor STAT5 signaling pathway activityScreen for activators or inhibitors of STAT5 signaling pathway
Description: The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line: Ba/F3
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Supplied As: Each vial contains ~3 x 10^6 cells in 1 ml of FBS with 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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