STAT5 Luciferase Reporter U937 Cell Line

Products from BPS Bioscience require a minimum order value above 400€

Applications: 1. Screen for activators or inhibitors of the STAT5 signaling pathway.
2. Screen for the neutralization antibody of GM-CSF.

Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine stimulating the production of granulocytes and monocytes from bone marrow precursors. GM-CSF had pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes.

Description: The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Host Cell Line: U937

Mycoplasma Testing: The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. 

Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-2

• SKU: BPS79941
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 79941
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human