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Encompassing Amino Acids: 2-488(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Ser/Thr Peptide 7, Invitrogen) at 30°C for 1 hour.
Background: MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20). Endogenous full-length MST1 is activated by a variety of stressful stimuli, accompanied by the secondary appearance of a 36 kDa Thr183-phosphorylated, caspase-cleaved catalytic fragment. Recombinant MST1 undergo a robust autoactivation in vitro, mediated by an intramolecular autophosphorylation on the activation loop of an MST dimmer. MST1 can initiate apoptosis when transiently overexpressed in mammalian cells. Interference with the ability of endogenous MST1 to associate with the putative tumor suppressor proteins Nore1/RASSF can inhibit Ras-induced apoptosis.
Description: Human STK4 (serine/threonine kinase 4), also known as MST-1 (Mammalian STE20-like protein kinase 1) or KRS2, GenBank Accession No. NM_006282, a.a. 2-488(end), with N-terminal GST-tag, MW=81.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol.
Genbank: NM_006282
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13043
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Taylor, L.K., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(19):10099-104.
2. Song, H., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(4):1431-6.
3. Ura, S., et al. (2001). Proc. Natl. Acad. Sci. USA. 98(18):10148-53.
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