Application Note: SUMO1 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. SUMO1 Protein is suitable for use in Western Blot. Expect a band approximately ~19kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 0.1 µg/µL
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant human SUMO1 (UBL1) (2-97) was expressed in E. coli using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.
Background: SUMO1 (also known as UBL1) is a member of the SUMO (small ubiquitin-like modifier) protein family which functions in a manner similar to ubiquitin. SUMO1 is bound to target proteins as part of a post-translational modification system and it is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (1). SUMO1 may be involved in the repair of TOP1-mediated DNA damage (2). The sumoylation pathway plays a significant role in mammalian DNA damage response. SUMO1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Apoptosis/Autophagy, Cell Cycle, and NfkB Pathway research.
Low Endotoxin: No
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