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Encompassing Amino Acids: 82-412
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and 10-100 ng SUV39H1) is added to the wells of a substrate-coated Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Description: Human SUV39H1, GenBank Accession No. NM_003173, a.a. 82-412, with N-terminal GST-tag, MW= 64 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Genbank: NM_003173
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O43463
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Kang, M.Y. et al., Int. J. Cancer 121, 10, 2192-2197, 2007.
2. Spensberger, D. and Delwel, R. FEBS Lett. 582, 18, 2761-2767, 2008.
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