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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Description: Recombinant full-length human SYK (GenBank accession #NM_003177) with an N-terminal GST-tag was expressed in Sf9 insect cells via a baculovirus expression system. MW = 10 kDa.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,3 mM DTT and 10% glycerol.
Genbank: NM_003177
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P43405
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.
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