Application Note: TAF1 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. TAF1 Protein is suitable for use in Western Blot. Expect a band approximately ~155kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 0.1 µg/µL
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant human TAF1 (1032-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
Background: TAF1 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. TAF1 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. TAF1 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition (1). TAF1 also serves to link the control of transcription to the cell cycle (2). TAF1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cell Cycle, and Invasion/Metastasis research.
Low Endotoxin: No
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