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Encompassing Amino Acids: 1517-1649
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: TAF1L (1517-1649) protein is incubated with biotinylated acetyl- histone peptide substrate (Cat. #33000) in TAF1L assay buffer in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, and the mixture is incubated for 30 minutes at room temperature (RT). AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer) are then added. After 10 minute incubation at RT, measure Alphacounts.
Description: Human TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, also known as TAF1L, GenBank Accession No. NM_153809, a.a. 1517 - 1649 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 42.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol.
Genbank: NM_153809
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8IZX4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wang, PJ., et al., Hum. Mol. Genet. 2002 Sep 15; 11(19): 2341-2346.
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