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Encompassing Amino Acids: TAK1 (1-303), TAB1 (437-end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a kinase buffer containing 0.2 mM DTT using MBP substrate (0.1 mg/ml) and 100 mM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using ADP-Glo® reagent (Promega).
Background: TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP) (1). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (Nf?B). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate Nf?B and JNK and are prone to apoptosis upon stimulation.
Description: Recombinant fusion protein consisting of human proteins TAK1 (TGFβ-activated kinase 1), also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7), GenbankAccession #NM_003188, a.a. 1-303, and human TAB1 (TAK1-binding protein), Genbank Accession #NM_006116, a.a. 437-end, linked by a small peptide (DFGGGGG), and with an N-terminal GST-tag, MW = 74 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Genbank: NM_003188, NM_006116
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O43318(TAK1), Q15750 (TAB1)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Yamaguchi, K. et al. Science 270: 2008-2011, 1995.
2.Liu, H.-H. et al. Proc. Nat. Acad. Sci. 103: 11677-11682, 2006.
3. Sakurai, H., et al. (2000). FEBS Lett.(Netherlands) 474 (2-3): 141-5.
4. Liu, H.-H., et al. (2006). Proc. Natl. Acad. Sci. USA 103: 11677-11682.
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