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Applications: Monitor Wnt /β-catenin signaling pathway activity.
Screen for activators or inhibitors of the Wnt /β-catenin signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the Wnt /β-catenin pathway.
Background: The Wnt/β-catenin signaling pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of β-catenin. β-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription andexpression of Wnt-responsive genes.
Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
Description: The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
Format: assay kit
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)
Supplied As: Reporter: TCF/LEF luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling intissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.
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