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Applications: Useful for the development of improved universal CAR-T or other effector cells.
Background: The T Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR triggers a signaling cascade that leads to the activation of transcription factors involved in the upregulation and secretion of cytokines, T cell proliferation, and cell differentiation into effector and memory cells. TCR-activated transcription factors include AP-1 (Activator Protein 1), NF-κB (Nuclear Factor Kappa-light-chain-enhancer of activated B cells) and NFAT (Nuclear Factor of Activated T-cells).Beta-2-Microglobulin is a required component of Major Histocompatibility Complex (MHC) class 1 molecules, which present peptide fragments from within the cell to cytotoxic T cells as part of the adaptive immune system. B2M plays an essential role both in governing MHC class I molecule stability and in promoting antigen binding by presenting the antigen to CD3/TCR complex of CD8+ T cells.Knockout of both TCR and B2M will support the manufacture of universal CAR-T cells. Knockout of TCR or B2M prevents the elimination of allogeneic T cells that express foreign HLA-I molecules, thereby enabling the generation of CAR-T cells from allogeneic healthy donors T cells in vivo.
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing.
Host Cell Line: Jurkat (clone E6-1), human T lymphoblast, suspension
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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