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Applications: Useful for the study of TDO enzymology, screening inhibitors and selectivity profiling.
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for proteinsynthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established asa key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue forimmune evasion.
Description: The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation activity is measured by measuring the absorption of reaction product at λ = 320-325 nm.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Warnings: DMSO >0.5%, strong acids or bases, ionic detergents, high salt
Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Li, J.S., et al. Arch Insect Biochem Physiol. 2007 Feb; 64(2): 74-87
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