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Encompassing Amino Acids: 2-406(end)
Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: Assay was performedby mixing 10 µl of varying concentrations ofmouse TDO2 with 170 µl of TDO ReactionSolution (BPS, #73005) and 20 µl of 1xTDO Assay Buffer (BPS, #73006). Thisreaction was incubated at room temperaturefor 90 min and absorbance was read at=320-325 nm.
Description: Mouse Tryptophan 2,3-dioxygenase-2 (TDO2), GenBank Accession No.NM_019911, a.a. 2-406(end) with an NterminalHis-tag, expressed in an E. coli cellexpression system. MW = 49 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 300 mM imidazole
Genbank: NM_019911
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P48776
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Pillotte, L., et al., Proc. Nat. Acad. Sci(USA) 2012; 109(7):2497-2502.
2. Miller, C.L., et al., Neurobiol. Disease2004; 15(3): 618-629.
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