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Encompassing Amino Acids: full length
Assay Conditions: Enzyme reaction was performed in areaction buffer (200 µl) containing 50 mMTris (pH 7.4), 1 mM MgCl2, 50 mM KCl, 1mM DTT, 0.1 mg/ml BSA, 2 mM T5PNP and600 ng TDP2. Reaction was continuouslymonitored at 415 nm at room temperature for20 min. Specific activity was calculated based on themolar extinction coefficient of the product (p-nitrophenol),e = 15,000 M-1cm-1
Description: Human tyrosyl DNA phosphodiesterase 2, or TDP2, (GenBank Accession No. NM_016614), full length with N-terminal His-GST-tag, MW = 67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol and 3 mM DTT
Genbank: NM_016614
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: O95551
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Loughney, K, et al., Int. J. Impot. Res.2005, 17(4):320-5
2. Weeks, JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9
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