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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: TET enzyme wascombined with a TET methylatedsubstratein assay buffer for 90 minutes.A fluorescent-conjugated antibody thatrecognizes hydroxymethylated productwas added and fluorescence was read atexcitation: 530 nm and emission 590 nm.
Description: Human Ten-eleven translocation 1protein (TET1), methylcytosinedioxygenase TET1, Leukemiaassociatedprotein with a CXXC domain,CXXC-type zinc finger protein 6,GenBank Accession # NM_030625, a.a.1418-2136(end) with N-terminal FLAG-tag,MW = 80 kDa, expressed in Sf9cells via a baculovirus expressionsystem.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 µg/ml FLAG peptide
Genbank: NM_030625
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q8NFU7
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Tahiliani, M., et al., Science. 2009; 324(5929): 930-935.
2. Guo, J.U., et al., Cell. 2011; 145(3): 423- 434.
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