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Encompassing Amino Acids: 824-1795 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human Ten-eleven translocation 3 protein (TET3), also known as methylcytosine dioxygenase TET3, GenBank Accession # NM_001287491, a.a. 824-1795 (end) with N-terminal FLAG-tag and C-terminal His-tag. MW = 108 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 140 mM Imidazole
Genbank: NM_001287491
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal FLAG-tag, C terminal His-tag
Uniprot: O43151
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tian-Peng, G., et al., Nature. 2011;477: 606-610.2. Abdel-Wahab, O., et al., Blood. 2009; 114: 144-147.