TetR HEK293 Cell Line

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Background: The T-REx cell line expresses the tetracycline repressor protein. Cells are transfected with the gene of interest under control of a minimal promoter and the tet operon. The Tet repressor protein binds to the operon and represses transcription. Tetracycline regulation is based on the binding of either tetracycline (or doxycycline) to the Tet repressor protein, which de-represses the promoter controlling expression of the gene of interest.

Description: Recombinant HEK293 cell transfected with tetracycline repressor gene. This cell line is validated for response to the stimulation of doxycycline.

Format: Each vial contains 1.5 X 10^6 cells in 1 ml of 10% DMSO

Host Cell Line: HEK293

Storage Stability: Store in liquid nitrogen immediately upon receipt.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-2

References: On the use of the T-REx tetracycline-inducible gene expression system in vivo. Dobrovolsky VN1, Heflich RH. 2007. Biotechnol Bioeng. 98(3):719-23.

• SKU: BPS71227
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 71227
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human