TGF-beta1(Transforming Growth Factor Beta 1) ELISA Kit

Abbreviation: TGF-β1

Target Synonym: Cartilage-inducing factor;CED;Differentiation inhibiting factor;DPD1;LAP;Latency-associated peptide;Prepro transforming growth factor beta 1;TGF beta 1;TGF beta;TGF beta 1 protein;TGF-beta 1 protein;TGF-beta-1;TGF-beta-5;TGF-beta1;TGFB;Tgfb-1;tgfb1;TGFB1;TGFbeta;TGFbeta1;Transforming Growth Factor b1;Transforming Growth Factor beta 1;Transforming growth factor beta 1a;transforming growth factor beta-1;transforming growth factor;beta 1;Transforming Growth Factor-ß1;

Target Species: Universal

Usage: This ELISA kit applies to the in vitro quantitative determination of TGF-β1 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

UNIProt ID: H:P01137;M:P04202;R:P17246

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal TGF-β1. You can calculate the concentration of Universal TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Universal TGF-β1 in samples.No significant cross-reactivity or interference between Universal TGF-β1 and analogues was observed

• SKU: ELSE-EL-0162-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-EL-0162-48
• Green Labware: No
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA