Description: Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. The kit is compatible with both suspension and adherent cells and is ideal for the pharmacological characterization and screening of Gs- and Gi-coupled GPCRs.
Components: cAMP standard (100 µL), Biotin-cAMP (25 µL), Eu-streptavidin (25 µL), Acc-anti-cAMP (50 µL), 5X cAMP Detection Buffer (2 x 1 mL).
Assay Type: Quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Low-volume 384-well plate.
Assay Target: cAMP.
Assay Target Class: Functional cAMP assay.
Detection method: TR-FRET.
Sample type: Cell lysates.