THUNDER Extreme Phospho-ERK1/2 (T202/Y204) + Total ERK TR-FRET Cell Signaling Assay Kit

Description: Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels.

Components: Eu-phospho-ERK (20 µL), Acc-phospho-ERK (80 µL), Eu-Total ERK (5 µL), Acc-Total ERK (20 µL), ERK control lysate (500 µL), 5X Lysis Buffer #1 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-ERK1/2 (T202/Y204) and Total ERK1/2.

Assay Target Class: Kinase.

SwissProt: P27361, P28482.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-XTM-ERKPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-XTM-ERKPT-500
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human, Mouse (Murine), Rat (Rattus)
• Application: Cell-based Assay
• Human Gene ID: 5595